Over the past two months my group has been working on hatching mosquitoes from eggs we collected over the summer. Unfortunately, none of our eggs have hatched. At first we assumed the eggs could simply be re hydrated but have now learned that is not the case. Mosquito eggs need nutrients in the water to be able to grow and eventually hatch.
We have had even more issue with our nutrient solution. We have tried three different solutions but so far none have been effective(all eggs remained unhatched and dissolved oxygen levels were extremely high). We have contacted Western North Carolina and are hoping they are able to help us create a successful nutrient broth so we can start our tests.
Once our mosquitoes have hatched we will run them through a y-tube olfactometer to see scent attractiveness to local flowers.
Our group has been collecting tardigrades from mosses that were collected in DuPont State Forest. We have collected from High Falls Parking Lot twice so far this year. Each time the moss has been damp from after a storm making it the perfect place to find tardigrades. There was more than enough moss in our collecting area. Each person in our group filled up at least two bags if not more.When we returned to the TIME room we were delighted to find that the moss we collected had an ample supply of tardigrades. The last of the tardigrades we had prior to our second collection had been running short. Now we have plenty of tardigrades to finish out our testing!
This year our group is working with tardigrades. Specifically the effects of glyphosate and imidacloprid on the tardigrades. So far we have done our control tests and are about to begin are glyphosate testing. In our control all the tardigrades in test two and test three survived. However in test one, three tardigrades died. This made our survival rate for the control 90%. It is possible that in test one the three tardigrades were dead when placed in the Petri dish. As we continue our experiment we assume that the survival rates for glyphosate and imidacloprid will be lower than 90%.