The search for lignin degrading enzymes is starting to come to a close. After identifying which of our samples were producing enzymes using the guaiacol assay, we performed PCR on these samples and sent the isolated DNA of to be sequenced. After receiving the sequenced DNA, we blasted the samples using the GENBANK database. Our results were surprising. Apparently, the green mold we had thought was contamination was actually a LDE producing fungi! I guess we should have done some more research on the fungi we had assumed was hurting our samples before we jumped to conclusions.
Now that we have identified our samples, were are moving into the project assembly phase. Much of the past few weeks had been spent creating our final presentation, writing our paper, and creating graphs. With Christmas break coming and our project coming to close, our group is experiencing the bittersweet feeling of the end coming into sight.